STANDARD OPERATING PROCEDURE FOR THE OPERATION OF LIQUID NITROGEN STORAGE TANK

1.0 PURPOSE

To design the standard operating procedure for the operation of the Liquid Nitrogen Storage Tank.

2.0 SCOPE

2.1 This SOP shall be applicable to the operation of the Liquid Nitrogen Storage Tank.

3.0 RESPONSIBILITY

3.1 All the users including Scientists (s) and Research associate(s) of the invitro department is responsible for the implementation of this SOP and maintaining the Liquid Nitrogen Storage Tank.

4.0 DISTRIBUTION

4.1 The Quality Assurance department is responsible to keep SOP ‘Master Copy’ approved through the Quality Manager.

4.2 The copy of ‘Control Copy’ of this SOP is being distributed in the invitro department and placed near related Equipment/ Instrument as ‘Display Copy’.

5.0 ABBREVIATIONS

5.1 Abbreviations

5.1.1 SOP: Standard Operating Procedure

6.0 DEFINITIONS

6.1 Definitions

6.2.1 Liquid Nitrogen Storage Tank is a cryo tank or cryogenic tank that is used to store frozen biological material.

7.0 PROCEDURE

7.1 Wear a face shield and loose-fitting insulated gloves when retrieving samples. Rapid changes in temperature can cause a rupture in a sample container causing containers to explode if liquid nitrogen has leaked inside.

7.2 Slowly lift the sample container straight up, then tilt slightly to allow excess liquid to run off before lifting the item fully out of the Dewar.

7.3 Gently set the sample container in a suitable location and retrieve the desired samples.

7.4 When returning the sample container to the Dewar, ensure the wire hook is affixed securely, then lower slowly into the Dewar to minimize splashing.

7.5 Replace the lid.

8.0 PRECAUTIONS

8.1 Wear goggles and a face shield if there is a risk of contact. Gloves should be loose and insulating. Loose gloves are recommended so that they can be thrown off in the event of a spill onto the gloves. Long sleeves, lab coats, or aprons, must be worn. Protective equipment is to be worn at all times.

8.2 Store in a cool dry place. If nitrogen has a blue tint, it has been contaminated with oxygen and should be replaced. The contaminated material is dangerous and potentially explosive.

8.3 If a release occurs, immediately stop all work. Rapid displacement of oxygen can occur. Alert other nearby workers and supervisors to the situation. Make sure no one has received hazardous exposure. If so, seek medical help immediately. If it can be done safely, shut off the flow and ventilate the area. This may not be possible in the event of a large spill. Avoid contact with liquid, and contact the Health and Safety Office and supervising staff members for spill response assistance.

8.4 Review the lab-specific SOP for information regarding emergency procedures and equipment. In the event of a large spill, consider pulling the building alarm, due to the asphyxiation hazard. Evacuate the area and alert others.

9.0 REFERENCES

9.1 Reference

9.1.1 User manual.

                                                                END OF THE DOCUMENT

You may like to read these links:   

1. List of All SOPs and Documents for In-vivo Laboratory

2. List of All SOPs and Documents for Study Protocol

3. List of Pharmacological Study Models with details about disease Inducing agents, Standard or Reference drugs, and related parameters.

4. List of OECD Guidelines or Toxicological Studies

5. List of Pharmacopeial Test or Quality Control Test or Batch Testing Parameters (In-vivo)

6. List of All SOPs and Documents for Calibration of the Instruments and Equipment

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