1.0 PURPOSE
To define the procedure for operation, maintenance, and cleaning of Biomedical Deep Freezer.
2.0 SCOPE
This Standard Operating Procedure (SOP) is applicable for the operation, maintenance, and cleaning of Biomedical Deep freezers for Biology studies.
3.0 RESPONSIBILITY
3.1 All personnel in the Biology Department shall be responsible for implementation and compliance with the SOP.
3.2 Head/Designees/Study Directors of the Biology department shall be responsible to ensure the implementation and compliance with the SOP.
4.0 DISTRIBUTION
4.1 The Quality Assurance department is responsible for keeping SOP ‘Master Copy’ approved by the Quality Manager.
4.2 The copy of ‘Control Copy’ of SOP is being distributed in the Biology department and placed near related Equipment/Instruments as Display Copy.
5.0 DEFINITIONS
5.1 Definition(s)
5.1.1 Deep Freezer is a stand-alone freezer unit for preserving foods in contrast to a freezer atop a refrigerator.
6.0 ABBREVIATIONS
6.1 Abbreviation(s)
6.1.1 SOP: Standard Operating Procedure
6.1.2 ˚C: Degree Celsius
6.1.3 RCF: Relative Centrifugal Force
6.1.4 RPM: Revolution per Minute
6.1.5 DEC: Display Energy Certificate
7.0 PROCEDURE
7.1 System description
7.1.1 Compressor
7.1.2 Cooling fan
7.1.3 Thermostat
7.1.4 Start relay
7.1.5 Sensor
7.1.6 Start Capacity
7.1.7 Power Plug
7.2 Introduction
A stand-alone freezer unit for preserving biomedical waste/dead bodies/organs in contrast to a freezer atop a refrigerator.
6.1 Abbreviation(s)
6.1.1 SOP: Standard Operating Procedure
6.1.2 ˚C: Degree Celsius
6.1.3 RCF: Relative Centrifugal Force
6.1.4 RPM: Revolution per Minute
6.1.5 DEC: Display Energy Certificate
7.0 PROCEDURE
7.1 System description
7.1.1 Compressor
7.1.2 Cooling fan
7.1.3 Thermostat
7.1.4 Start relay
7.1.5 Sensor
7.1.6 Start Capacity
7.1.7 Power Plug
7.2 Introduction
A stand-alone freezer unit for preserving biomedical waste/dead bodies/organs in contrast to a freezer atop a refrigerator.
Fig 1: Biomedical Deep Freezer Setup
7.3 Principle
The basic working principle of a freezer is evaporation. When a liquid evaporates it causes the surrounding area to cool. A vapor compression cycle is used in most freezers. In this cycle, a circulating refrigerant enters a compressor as low-pressure vapor at or slightly above the temperature of the freezer interior. The vapor is compressed and exits the compressor as high-pressure superheated vapor. The superheated vapor travels under pressure through coils or tubes that make up the condenser; the coils or tubes are passively cooled by exposure to air in the room. The condenser cools the vapor, which liquefies. As the refrigerant leaves the condenser, it is still under pressure but is now only slightly above room temperature. This liquid refrigerant is forced through a metering or throttling device, also known as an expansion valve (essentially a pin-hole-sized constriction in the tubing) to an area of much lower pressure. The sudden decrease in pressure results in explosive-like flash evaporation of a portion (typically about half) of the liquid. The latent heat absorbed by this flash evaporation is drawn mostly from the adjacent still-liquid refrigerant, a phenomenon known as auto-refrigeration. This cold and partially vaporized refrigerant continues through the coils or tubes of the evaporator unit. A fan blows air from the freezer compartment ("box air") across these coils or tubes and the refrigerant completely vaporizes, drawing further latent heat from the box air. This cooled air is returned to the freezer compartment, and so keeps the box air cold.
Fig 2:
Circuit Diagram
7.4.1 Model:.............
7.4.2 Environment temperature: 10~32 oC
7.4.3 Inside temperature: -10~-40 oC
7.4.4 Effective volume:...........
7.4.5 Rated voltage:............
7.4.6 Rated frequency:.........
7.4.7 Input power:..........
7.4.8 Refrigerant: .............
7.4.9 Outline dimension: ..............
7.5 Placing environment
7.5.1 The freezer should be placed stably and the packing base should be removed for reducing shake and noise. When it is carried or moved, the slanting angle should be larger than 45 oC.
7.5.2 The freezer should be placed away from the heat source and direct sunshine, and the environment temperature should not be higher than 32 oC.
7.5.3 Space should have remained around the freezer for ventilation and heat radiation. Do not place the freezer in a very cold frozen environment.
7.5.4 Do not place the freezer in a humid environment.
7.5.5 Do not place that often suffers water splash, and the splashed water or sewage must be wiped out by soft cloth in time.
7.5.6 Rain drop is forbidden in the freezer.
7.6 Preparation before use
7.6.1 The foam base should be removed.
7.6.2 Please check the items with the content of the “packing list”.
7.6.3 Freezer should be cleaned before use.
7.6.4 Ever time after carrying, the freezer has to be placed without any move for 30 minutes before power-on.
7.6.5 Power the freezer on for test operation; if an obvious cooling effect has been produced after half an hour, it is a sign of normal working.
7.6.6 Take out low screw hole covers on the left side of the freezer door, mount the door handle, and screw the bolt.
7.7 Starting up operation and temperature adjustment
7.7.1 Starting up operation
Let the freezer run without any products inside for some time (about 6h in summer, 4h in winter), then put the products into the freezer for the store.
7.7.2 Temperature adjustment
The electronic temperature controller has been installed on the control panel. When power is on, the digital screen can show the inside temperature. The following is the electronic temperature sketch map.
Figure 3: Temperature
Adjustment Display
When powered on, the screen shows the present temperature value, if a temperature reset is needed, for example, adjust the pre-set value from -30 oC to -35 oC, and please follow the steps below:
Press “˄”, “˅” keys for 3 seconds simultaneously and the set temperature begins flickering. Whenever the “˅” key is pressed down, the temperature will drop 1 oC until -40 oC; Whenever the “˄” key is pressed down, the temperature will increase 1oC until -10 oC, then press back to -40 oC; if no operation is done in 5 seconds, then it exits the set state to resume the normal temperature display.
7.7.3 Temperature alarming
For the safety of the inside box goods, the electronic temperature controller has an alarming function. When the inside temperature exceeds the alarming upper limit or lower limit, the buzzer will give an alarm, and the alarming indicator light on the screen will flicker at the same time. Press the release key to stop the buzzing (the alarming light will keep flickering). When the inside temperature is within the range of upper and lower limits, the buzzing will disappear automatically.
7.7.4 Over-high temperature alarming function
When the freezing sensor shows the actual temperature above -15 Celsius the over-high temperature alarm indicator light will always be on with the sound of buzzing (buzzing once per minute, three buzzers in one time at 0.5 seconds interval), press down the buzz release key to stopping the buzzing while the red light keeps on (even more press will not work out to resume the buzzing sound, and only if the display temperature reduces to under -15 oC or power-off, the buzzing release key will automatically reset and the buzzing function will resume).
Notes: When the freezing sensor faults occur, the red light will be always on and the alarming light will lose function.
7.7.5 Supply interruption memory function
When powered again after supply interruption, the low-temperature freezer has a memory function so that it can keep on working under the setting condition before the supply was interrupted.
7.7.6 Errors on display
When there is a short, broken circuit or breakdown of the temperature sensor, the temperature display shows “F1”; the low-temperature freezer enters the protective mode. In this situation, the compressor falls into a fixed recycle of starting up for 20 minutes and stopping for 5 minutes, the red light keeps on the alarming sound does not work.
Suggestion: When judging the temperature sensor is in the wrong condition, please cut off power first and check if the connecting wire of the sensor is loose.
7.7.7 Attentions
The temperature of the top drawer is 2-3 C higher than that of other drawers, so you’d better store the goods which have a strict storing temperature requirement in other drawers except the top one.
If the freezer stop running due to power failure or other faults, the inside temperature will go up. If the power cannot be supplied in a short term, please take out the goods for other low-temperature stores lest loss on the stored goods.
Before putting goods into the freezer for low-temperature storage, please confirm if the freezer can meet the low-temperature requirement you have set in advance lest any loss occurs resulting in the stored goods being damaged due to the inappropriate temperature of the freezer.
7.8 Defrosting/stopping use
7.8.1 After using it for some time, the evaporator surface will form a frost. If the layer is too deep, it will impair the cooling effect and increase the power consumption. So at a period interval, when the depth of the frost layer reaches about 10 mm, a plastic shovel should be used to remove it.
7.8.2 The inside goods should be withdrawn before defrosting. Place them in another freezer for the store. When defrosting place the freezer in ventilation with the door open.
7.8.3 After the frost layer has been melted, sop water out with a soft cloth or sponge, wipe the freezer with a dry cloth, then plug it on, and put the goods in until reaching the set temperature.
7.8.4 To expedite the defrosting progress, a flat container containing hot water with a temperature below 50 ˚C can be put inside the freezer.
7.8.5 Stopping use: If the freezer is not in use for a long time, power should be cut off, clean it as following methods, and after air dry with the cover open, seal it for the store.
7.8.6 Attention: Do not use the electric heater for defrosting, and do not use metal tools or sharp tools for defrosting.
7.9 Cleaning and maintenance
7.9.1 For safety, the freezer should be unplugged before cleaning.
7.9.2 When cleaning the freezer, a soft cloth should be used, sopped sponge, or soap (any non-corrosive neutral abluent). After cleaning up, wipe the freezer with a dry cloth preventing it from rusting.
7.9.3 Dust should be often erased from the condenser, the compressor, and other pipelines with a soft brush to keep a good cooling effect.
7.9.4 Organic dissolvent should not be used, hot water, laundry soap powder, or acid material to clean the freezer.
7.9.5 When cleaning the freezer, water should not be used to wash the freezer; do not use a hard bristle brush, or wire brush to clean the freezer.
8.0 PRECAUTIONS
8.1.1 To ensure the safe operation of the Biomedical Deep Freezer, the following general safety regulations must be followed:
8.1.1.1 The Deep Freezer uses Ac ....V/50/...Hz power supply. If the working voltage is below 198V or above 242V, an automatic voltage regulator with 1000W or above should be installed for adaptation. If the power wire needs to be lengthened, the cross-section of the extended wire should not be smaller than 1 mm2.
8.1.1.2 Special socket should be used for ground connection. The power wire of the freezer has been connected with a three-way plug (ground connection) which is by the three-plug connector (ground connection) standard. On any condition, do not remove or cut the third plug of the power wire (ground connector). After the placement of the freezer, the plug could be touched.
8.1.1.3 It is strictly forbidden for the storage of inflammable and explosive articles and strongly corrosive acid, alkali, etc.
8.1.1.4 Please do not use the inflammable pressured spray near the freezer for fear of fire.
8.1.1.5 When inflammable gas like coal gas is leaking, please:
8.1.1.5.1 Shut down the leaking valve;
8.1.1.5.2 Open the door and window for ventilation
8.1.1.5.3 Do not plug or unplug the freezer
8.1.1.6 Do not let children play nearby for fear of accidents.
8.1.1.7 Once the freezer is cut off, please wait for more than 5 minutes for the next power-on test the compressor or system could be damaged. For protection and maintenance, please unplug the freezer. Please do not roll or damage the power wire.
8.1.1.8 Please wear protective articles to protect from cold injury when storing or fetching the goods from the freezer.
9.0 REFERENCES
9.1 Reference
9.1.1 Company Deep Freezer Service Manual.
END
OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for In-vivo Laboratory
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for Animal House Facility
4. List of All SOPs and Documents for Laboratory Instruments and Equipment
Hi. I’m Writer of Researchsop.com. ’ ’ Please share these SOPs to all concern pharma people for their development. I like to fullfill the need of curious people. These things inspire me to make things looks better.
0 comments:
Post a Comment