1.0 Purpose
This Standard Operating Procedure
(SOP) outlines procedures for treating pinworm infestations in rodent colonies.
2.0 Responsibilities
2.1 Veterinarian:
- Diagnose pinworm infestation and prescribe treatment.
- Determine treatment duration (5 or 7 cycles, 9
or 13 weeks).
- Conduct post-treatment tests.
2.2 Animal Facility Manager:
- Inform investigators, users, and staff of
infestation, treatment, and special procedures.
- Ensure treatment adherence to this SOP.
2.3 Animal Care Attendant:
- Supply medicated feed/water according to the
prescribed schedule.
3.0 Materials
- Barrier facilities: Irradiated medicated feed
containing 22% or 150 ppm fenbendazole.
- Conventional facilities: Regular medicated feed
containing 22% or 150 ppm fenbendazole.
- Alternative: Ivermectin sheep drench 0.08%.
4.0 Procedures
4.1 Movement Restriction:
- Limit animal transfers out of the positive room
(unless the receiving room is under treatment).
- Receiving animals in positive rooms remains
permitted.
4.2 Treatment Initiation:
- Begin treatment at staff convenience, minimizing
research disruption.
4.3 Treatment Scope:
- Barrier facilities: Treat the positive room and
potentially exposed rooms.
- Conventional facilities: Consider facility-wide
treatment.
4.4 Fenbendazole Medicated Feed:
- Provide medicated feed ad libitum on weeks
1, 3, 5, 7, 9, (11), (13).
- Offer regular feed on weeks
2, 4, 6, 8, 10, (12), (14).
- (*Treat for a minimum of 5 cycles, ideally 7.)
- Supply only 7-day portions to minimize waste.
- Leave uneaten medicated feed; supplement with regular
feed for the remaining 7 days.
4.5 Ivermectin Medicated Water:
- Prepare by mixing 1 part ivermectin drench with 99
parts water.
- Conduct a small-scale test for adverse reactions
before full implementation.
- Follow the same schedule as with medicated feed (7
days on, 7 days off).
4.6 Animal Husbandry:
- Attend infected animals last, wearing gloves and
designated gowns.
- Barrier facilities: No autoclaving of soiled
cages or regulated medical waste disposal is required.
- Conventional facilities: Consider occasional
cage autoclaving to break the recontamination cycle.
- Dispose of dirty bedding carefully to minimize
aerosols and contamination.
4.7 Post-treatment Monitoring:
- Wait at least 3 months before declaring a room
negative.
- Randomly test 10-20 weanling cages per room using
PCR.
5.0 Documentation
- Maintain records of treatment dates, medications
used, and test results.
6.0 Training
- Train animal care staff on pinworm
identification, treatment procedures, and safety protocols.
7.0 References
- Include relevant references or SOPs for detailed
medication preparation and handling.
END OF THE DOCUMENT
You may like to read these links:
1. List of All SOPs and Documents for the Microbiology Laboratory
2. List of All SOPs and Documents for In-vitro Laboratory
3. List of All SOPs and Documents for the In-vivo Laboratory.
4. List of All SOPs and Documents for Clinical Research
5. List of Instruments and Equipment Used In Laboratory Animal Facility
6. List of All SOPs and Documents for the Animal House Facility
7. List of Guidelines for Toxicology Animal Studies
8. Checklist for Toxicology (OECD-407) study preparation for Histopathology
9. List of SOPs and Documents for Histopathology Laboratory
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