SOP FOR PROTECTING PEOPLE AND ANIMALS: WORKING WITH BSL-2 BIOHAZARDS IN RODENT STUDIES

1.0 Purpose:

This SOP outlines how to house and care for rodents exposed to biosafety level 2 (BSL2) biohazards. It helps to:

Contain the biohazards
Reduce the risk of exposure for people and other animals

2.0 Responsibilities:

Principal investigator:

Provides information about the biohazard in the Animal Study Protocol (ASP)
Gives a copy of the biosafety certificate to the Facility Supervisor
Makes sure research staff is trained before doing BSL2 experiments

Test Facility Manager:

Make sure the animal room and equipment are suitable for BSL2 experiments
Provides necessary materials
Makes sure staff and users are trained properly
Posts information for identifying risks

Research and animal care staff:

Follows procedures to minimize risks as described in this SOP

3.0 Materials:

Personal protective equipment (PPE): gown, gloves, sleeve covers, shoe covers, bonnet, mask, goggles or safety glasses
Biological Safety Cabinet (BSC)
Microisolator cages
Biohazard MSDS
Biohazard door sign
Biohazard waste containers/bags
Sharps container
Disinfectant
Dedicated materials and equipment (notebooks, cage cards, pens, ear punchers, instruments)

4.0 Procedures:

Wear proper clothing: long pants, socks, closed shoes.
Wear PPE when handling infectious materials or animals:

Bonnet covering all hair
Mask covering mouth and nose
Disposable gown, tied in the back
Double gloves, stretched over gown cuffs
Disposable sleeve covers, over gloves
Protective eyewear (recommended)

Use dedicated materials and equipment.
House animals in micro isolator cages or isolators.
Label cages with a biohazard sign, biohazard name, and administration date.
Do all procedures in a BSC (changing cages, infecting animals, necropsy, etc.).
Decontaminate work surfaces after use or spills with disinfectant.
Don't eat, drink, or store food in animal rooms.
Wash or sanitize hands after handling infectious materials or animals, before leaving the room.
Minimize creating aerosols during procedures.
Don't recap needles; dispose of them in a sharps container.
Have a vermin control program in BSL2 animal rooms and the rest of the facility.

5.0 Changing cages:

Do it in the BSC. Dispose of feed, water, and bedding as biohazard waste.
Bag dirty cages in an autoclavable bag under the BSC.
Seal the bag with autoclavable bands or ties before removing it.
Spray the bag with disinfectant before moving it to a second autoclavable bag.
Seal the second bag with bands or ties and spray with disinfectant.
Autoclave the cages and check for proper steam penetration.
Wait for weekly biological indicator results.
After autoclaving, dispose of bedding as regular waste and wash cages according to SOP.

6.0 Animal room:

Keep doors closed when infected animals are present.
Set the room to negative pressure compared to the corridor or anteroom.
Limit access to authorized personnel and users.
The facility manager must:

Post a biohazard sign with the biohazard name, medical requirements (if any), contact information, and PPE requirements.
Make sure personnel and users are properly informed about the biohazard and procedures.

Dispose of infectious waste and animal carcasses in double biohazard bags or containers.

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