1.0 Purpose:
This SOP applies to all personnel involved in the collection, handling, and analysis of drainage water samples at [Facility/Organization Name].
3.0 Responsibilities:
Designating a responsible person for sample collection and processing.
Appointing a trained laboratory analyst for sample analysis.
Ensuring equipment calibration and maintenance.
4.0 Materials and Equipment:
List all the materials and equipment required for the microbial monitoring process, including but not limited to:
Sterile sample containers.
GPS devices (if required for sample location tracking).
Personal protective equipment (PPE).
Ice packs or coolers for sample transportation.
Laboratory analysis equipment (incubators, petri dishes, etc.).
5.0 Sample Collection:
Detail the procedures for collecting drainage water samples, including:
Identifying sampling points and locations.
Frequency of sampling (daily, weekly, monthly, etc.).
Sample volume and collection containers.
Sterilization and handling of sample collection equipment.
Techniques to prevent sample contamination.
Sample Transportation and Storage:
Outline the steps for transporting and storing drainage water samples safely and efficiently, including:
Use of coolers or ice packs for maintaining appropriate temperature during transportation.
Labeling samples with unique identifiers for accurate tracking.
Storage conditions and duration before analysis.
To collect samples for microbiological testing, follow these steps:
1. Using a sterile pipette, individually sample approximately 20ml of drain water from each drain of the plant, both before and after sanitization. Transfer the collected samples to separate sterile beakers.
6.0 Laboratory Analysis:
Describe the laboratory analysis procedures for microbial monitoring, including:
Enumeration and identification of target microorganisms.
Techniques for sample inoculation and incubation.
Interpretation of results and reporting criteria.
6.1Total Viable Count Testing Procedure:
Prepare First Dilution (1:10 dilution):
Take 1 ml of drain water as a sample and mix it with 10 ml of sterile Peptone water or 0.9% normal saline solution in a sterile container.
Prepare Second Dilution (1:100 dilution):
Take 1 ml of the previously prepared 1:10 dilution and mix it with 100 ml of sterile Peptone water or 0.9% normal saline solution in another sterile container.
Prepare Third Dilution (1:1000 dilution):
Take 1 ml of the previously prepared 1:100 dilution and mix it with 1000 ml of sterile Peptone water or 0.9% normal saline solution in a separate sterile container.
Aseptically transfer 1 ml of the sample from each dilution to three different sterilized Petri plates.
Add approximately 20 ml-25 ml of sterilized molten Soybean Casein Digest Agar to one plate for bacterial growth, and Sabouraud Dextrose Agar to another plate for yeast and mold growth
After adding the culture media, gently swirl each plate to ensure proper mixing of the sample and agar, and allow the media to solidify.
Before incubation, label each plate with the location number and date.
6.2 Incubate the Petri plates:
Incubate the Soybean Casein Digest Agar plate at 30°-35°C for 2-3
days to encourage bacterial growth.
Incubate the Sabouraud Dextrose Agar plate at 20°-25°C for 5 days
to encourage yeast and mold growth.
Once the incubation period is complete, examine the plates for microbial growth in terms of colony-forming units (CFU).
Count the number of CFUs on each plate and calculate the average CFU from two plates to determine the concentration of viable microorganisms in the original drain water sample in terms of CFU/ml.
6.3 Microbial Limit Test for E.coli & Salmonella:
Prepare 1:1 Dilution:
Take 10ml of drain water and add it to 10ml of sterile Peptone water or 0.9% normal saline solution.
Prepare 1:10 Dilution:
Take 10ml of drain water and add it to 100ml of sterile Peptone water or 0.9% normal saline solution.
Prepare 1:100 Dilution:
Take 10ml of drain water and add it to 1000ml of sterile Peptone water or 0.9% normal saline solution.
Aseptically transfer 1 ml of the sample from each dilution to three different sterilized Petri plates.
Add approximately 20 ml-25 ml of sterilized Bismuth Sulfite Agar to one plate for Salmonella testing and MacConkey Agar to another plate for E.coli testing.
Mix the sample and culture media thoroughly and allow them to solidify.
Before incubation, label each plate with a location number and date.
Incubate the Bismuth Sulfite Agar plate at 37°C for Salmonella and the MacConkey Agar plate at 37°C for E.coli for 24-48 hours.
After the incubation period, observe the plates for microbial growth in terms of colony-forming units (CFU).
Count the number of CFUs on each plate and calculate the average CFU from two plates to determine the concentration of viable microorganisms in the original drain water sample in terms of CFU/ml.
6.4 Calculation:
Formula for Calculation: CFU/ml = Number of CFU Observed × Dilution Used (ml)
For 1:10 Dilution: CFU/ml = N × 10
For 1:100 Dilution: CFU/ml = N × 100
For 1:1000 Dilution: CFU/ml = N × 1000
Frequency: Perform the test once a month.
7.0 Data Recording and Management:
Establish guidelines for data recording and management, covering:
Maintaining a detailed record of sample collection and analysis dates, times, and locations.
Digital storage of data (if applicable).
The retention period for data and records.
8.0 Quality Control and Assurance:
Define the quality control measures to be implemented during the microbial monitoring process, such as:
Regular calibration and maintenance of laboratory equipment.
Proficiency testing and validation of laboratory methods.
9.0 Corrective Actions:
Specify the steps to be taken if microbial hazards or deviations from acceptable limits are detected, including:
Alerting relevant personnel and management.
Initiating appropriate corrective actions and investigations.
10: Safety Considerations:
Address safety measures to be followed during sample collection, transportation, and analysis, including:
Proper use of personal protective equipment (PPE).
Handling hazardous materials, if any.
Emergency response protocols in case of accidents or spills.
11.0 Training and Competency:
Outline the requirements for personnel training and competency assessment related to microbial monitoring procedures.
12.0 Revision History:
Maintain a revision history table to track changes to the SOP over time, including dates and descriptions of revisions.
13.0 References:
List all relevant regulations, guidelines, and references used to develop this SOP.
14.0 Approval:
Provide a section for designated personnel to sign and date the SOP to indicate their approval and acceptance of the document.
Once this SOP is completed, ensure that all relevant personnel receives proper training on its contents and that it is readily accessible to anyone involved in the microbial monitoring of drainage water at your facility or organization. Regular reviews and updates should be conducted to reflect changes in regulations or best practices.
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